We are thrilled to announce our upcoming Training School on Prime Editing (PE) applications and analysis, hosted at CIEMAT: Centro de investigaciones energéticas medioambientales y tecnológicas, in Madrid (Spain)
Image credit Shuto et al 2024 (Shuto, Y., Nakagawa, R., Zhu, S. et al. Structural basis for pegRNA-guided reverse transcription by a prime editor. Nature 631, 224–231 (2024). https://doi.org/10.1038/s41586-024-07497-8)
WHEN?
SEPTEMBER 1-2
Tuesday September 1st
| 08:30 | Welcome |
| 09:00 | PRIME EDITING – INTRODUCTION AND OVERVIEW Beatriz Olalla |
| 09:20 | PRIME EDITING – HOW DOES IT WORK? Johannes Karl Dohr |
| 10:00 | PRACTICAL SESSION: PEmax mRNA Part I: • Introduction: Steps to perform a Prime editing experiment. • Hands on: In vitro transcription of PEmax mRNA. |
| 12:00 | GUIDE DESIGN TOOLS Sofia Ottonello |
| 12:45 | LUNCH BREAK |
| 14:00 | PRACTICAL SESSION: PEmax mRNA Part II: • Hands on: DNAse treatment • Hands on: Poly(A) tail addition |
| 15:00 | PRACTICAL SESSION: PegRNA and sgRNA design Sofia Ottonello |
| 16:00 | PRACTICAL SESSION: PEmax mRNA Part III: • Hands on: mRNA purification • Hands on: mRNA quantification • Hands on: mRNA quality Control – Qubit quantification |
| 18:00 | Questions & Answers |
| 20:00 | Networking event |
Wednesday September 2nd
| 09:00 | PRIME EDITING APPLICATIONS: • Epidermolysis Bullosa – Alex Bassons • Fanconi anemia – Beatriz Olalla • Cardiomyopathies – Johannes Karl Dohr & Sofia Ottonello |
| 11:00 | PRACTICAL SESSION: PE system nucleofection: – Introduction: Experimental workflow: From electroporation to PCR analysis • Hands on: Nucleofection in suspension cells • Hands on: Nucleofection in adherent cells |
| 14:00 | LUNCH BREAK |
| 15:30 | PRIME EDITING SAFETY: OFF-TARGET ACTIVITY Sergio López |
| 16:00 | PRACTICAL SESSION: • Introduction: Next steps and data analysis • Hands on: CRISPResso analysis |
| 17:30 | Questions & Answers Adjourn |
Application & Funding
GenE-HumDi courses are open to all EU members and Near Neighbour Countries, with a particular focus on promoting the participation of Inclusiveness Target Countries and encouraging Early career scientist.
- Cost: Selected attendees are enrolled free of charge.
- Limited Capacity: Seats are strictly limited to ensure high levels of interaction between trainers and trainees.
- Funding: A call is now open to provide financial support for travel costs and daily allowances for selected participants, in accordance with COST guidelines.
A call is opened to provide funding for selected participants, to cover Travel costs and Daily Allowances, in accordance with the COST guidelines https://www.cost.eu/uploads/2025/11/COST-094-21-Annotated-Rules-for-COST-Actions-Level-C-V3.0-25-IX-2025.pdf
Important Considerations
Only applications received before July 14th will be considered for funding.
Applicants need to send the form bellow to raquel.soriano@juntadeandalucia.es
These forms will be used to shortlist applicants in case they exceed the seats available.
Granada, June 23, 2026
